Table 1.
The effect of different enzyme preparations on the relative mean fluorescence intensity (rMFI)* of leukocyte surface antigens in human stromavascular cells freshly isolated from adipose tissue following a 45–75 min digestion.
| Cell population | Surface Marker | Collagenase I (1 mg/ml) mean (SD) N=10 |
Liberase (0.035 mg/ml) mean (SD) N=8 |
p value |
|---|---|---|---|---|
| Leukocytes (CD45pos) | CD45 | 3,771 (553) | 3,992 (771) | 0.489 |
|
| ||||
| Neutrophils (CD15posCD16hi) | CD16 | 123,951 (75,778) | 62,066 (43,961) | 0.058 |
|
| ||||
| Macrophages (CD14posCD206pos) | CD14 | 4,237 (1,892) | 4,972 (1,598) | 0.146 |
| CD206 | 6,038 (1,538) | 5,143 (1,476) | 0.397 | |
| CD11c | 310 (141) | 318 (124) | 0.696 | |
| CD40 | 686 (413) | 565 (311) | 0.502 | |
| CD16 | 738 (671) | 1051 (774) | 0.372 | |
|
| ||||
| T lymphocytes (CD3pos) | CD3 | 1,423 (856) | 1,664 (831) | 0.558 |
| CD4 | 667 (390) | 90 (37) | <0.001 | |
| CD8 | 2,389 (1,269) | 1,584 (1,139) | 0.182 | |
|
| ||||
| Lymphocytes | CD16 | 21,117 (21,450) | 18,476 (16,418) | 0.897 |
*
rMFI was determined by subtracting the MFI of the isotype controls from the MFI for each population identified by a defined gate using specific cell surface markers. MFI itself was obtained using the geometric mean statistic.