FIGURE 5.
Altered regulation of ZEB1 and miR-200 are required for 2-HG-mediated induction of EMT-like phenotypes. A, Western blot of HCT116 IDH1 R132H/+ clone 2H1, HCT116 IDH1 R132C/+ clone 3A4, and HCT116 IDH2 R172K/+ clone 47C2 transfected with NTC siRNAs or one of three siRNAs targeting ZEB1 (A, B, or C) for 72 h. B, representative images of cells treated as in A are shown. Scale bar, 50 μm. C, Western blot of HCT116 IDH1 R132H/+ clone 2H1, HCT116 IDH1 R132C/+ clone 3A4, and HCT116 IDH2 R172K/+ clone 47C2 transfected with the indicated miRNA mimetics for 72. D, representative images from cells treated as in C. Scale bar, 50 μm.