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(A) McA-RH7777 rat hepatoma cells were cultured in medium containing 10% fetal bovine serum (normal medium) or in medium from which the lipids had been extracted and cholesterol added back at 10 μg/ml as a cyclodextrin complex (DLPS+C). Cells were stained for lipid with oil red O at 10 days post-transfection. (B) Immunoblot of POR and CD36 expression in control (Co) and POR-suppressed cells (siRNA) after 5 days. M, mass markers at 100 and 70 kDa.
