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. Author manuscript; available in PMC: 2013 Jan 9.
Published in final edited form as: Sci Signal. 2010 Aug 10;3(134):ra60. doi: 10.1126/scisignal.2001104

Fig. 4.

Fig. 4

Enhanced VDJH recombination in the absence of PI3K signaling. FISH analysis of intergenic sequences in the Igμ locus. (A) Representative images of cells incubated with a probe that hybridizes between the VH and DH loci (green) or within the CH locus (red). The image on the left shows a cell with both loci intact. The image in the middle shows a cell that has lost the inter VH-JH segment (that is, it has undergone VH to DJH recombination) on one allele, whereas the image on the right shows a cell that has undergone VH to DJH recombination on both alleles. (B) Percentages of p110αfl or p110αflδD910A cells with zero (red), one (blue), or two (yellow) VDJH recombined alleles. More than 200 cells from two independent experiments were scored. (C) The ratio of cells that had undergone VH to DJH rearrangement on one versus two alleles is shown.