Abstract
A technique has been described for selection of bacteria carrying plasmid λdv. With this technique, the effects of mutations in the promoter-operators were compared on the production and perpetuation of the plasmid. It was found that “left” promoter-operator that controls leftward gene expressions can be deleted from the plasmid genome. Some mutations of “right” promoter-operator (pRoR) that controls expression of genes tof, O, and P affect the stability of the plasmid. However, the plasmid genome accomodates a variety of pRoR mutations within a reasonable but different degree of constitutivity. Some new promoter mutations that allow bypass of the pRoR cannot be carried in the plasmid genome. From these findings it was proposed that the plasmid replicon has one indispensable promoter-operator that controls expression of all the genes related to its own replication, although a variety of constitutive mutations can be accommodated in the pRorR.
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- Arber W., Wauters-Willems D. Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification systems of strain 15T-. Mol Gen Genet. 1970;108(3):203–217. doi: 10.1007/BF00283350. [DOI] [PubMed] [Google Scholar]
- Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Echols H., Green L., Oppenheim A. B., Oppenheim A., Honigman A. Role of the cro gene in bacteriophage lambda development. J Mol Biol. 1973 Oct 25;80(2):203–216. doi: 10.1016/0022-2836(73)90167-8. [DOI] [PubMed] [Google Scholar]
- Eshima N., Fujii S., Murotsu T., Horiuchi T. Lambda phage mutants insensitive to temperature-sensitive repressor. 3. Effects of virL, virR or virC mutation on the gene expression. Mol Gen Genet. 1972;116(1):84–87. doi: 10.1007/BF00334262. [DOI] [PubMed] [Google Scholar]
- Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
- Hampacherová M., Koutecká E., Neubauer Z. Two repressors in bacteriophage lambda. Mol Gen Genet. 1973 Jan 24;120(2):133–137. doi: 10.1007/BF00267241. [DOI] [PubMed] [Google Scholar]
- Helinski D. R., Clewell D. B. Circular DNA. Annu Rev Biochem. 1971;40:899–942. doi: 10.1146/annurev.bi.40.070171.004343. [DOI] [PubMed] [Google Scholar]
- Ikeda H., Tomizawa J. Prophage P1, and extrachromosomal replication unit. Cold Spring Harb Symp Quant Biol. 1968;33:791–798. doi: 10.1101/sqb.1968.033.01.091. [DOI] [PubMed] [Google Scholar]
- JACOB F., SUSSMAN R., MONOD J. [On the nature of the repressor ensuring the immunity of lysogenic bacteria]. C R Hebd Seances Acad Sci. 1962 Jun 13;254:4214–4216. [PubMed] [Google Scholar]
- KAISER A. D. A genetic study of the temperate coliphage. Virology. 1955 Nov;1(4):424–443. doi: 10.1016/0042-6822(55)90036-2. [DOI] [PubMed] [Google Scholar]
- KAISER A. D., JACOB F. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology. 1957 Dec;4(3):509–521. doi: 10.1016/0042-6822(57)90083-1. [DOI] [PubMed] [Google Scholar]
- KAISER A. D. Mutations in a temperate bacteriophage affecting its ability to lysogenize Escherichia coli. Virology. 1957 Feb;3(1):42–61. doi: 10.1016/0042-6822(57)90022-3. [DOI] [PubMed] [Google Scholar]
- Koga H., Miyauchi T., Horiuchi T. Lambda phage mutants insensitive to temperature-sensitive repressor. II. Genetic character of lambda vir C mutant. Mol Gen Genet. 1970;106(2):114–122. doi: 10.1007/BF00323830. [DOI] [PubMed] [Google Scholar]
- Liedke-Kulke M., Kaiser A. D. Genetic control of prophage insertion specificity in bacteriophages lambda and 21. Virology. 1967 Jul;32(3):465–474. doi: 10.1016/0042-6822(67)90298-x. [DOI] [PubMed] [Google Scholar]
- MATSUSHIRO A. Specialized transduction of tryptophan markers in Escherichia coli K12 by bacteriophage phi-80. Virology. 1963 Apr;19:475–482. doi: 10.1016/0042-6822(63)90041-2. [DOI] [PubMed] [Google Scholar]
- Matsubara K. M. Interference in phage growth by a resident plasmid lambda dv. I. The mode of interference. Virology. 1972 Dec;50(3):713–726. doi: 10.1016/0042-6822(72)90425-4. [DOI] [PubMed] [Google Scholar]
- Matsubara K. Interference in bacteriophage growth by a resident plasmid lambda dv. II. Role of the promoter-operator. J Virol. 1974 Mar;13(3):603–607. doi: 10.1128/jvi.13.3.603-607.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsubara K., Kaiser A. D. Lambda dv: an autonomously replicating DNA fragment. Cold Spring Harb Symp Quant Biol. 1968;33:769–775. doi: 10.1101/sqb.1968.033.01.088. [DOI] [PubMed] [Google Scholar]
- Matsubara K. Plasmid formation from bacteriophage lambda as a result of interference by resident plasmid lambda dv. Virology. 1972 Mar;47(3):618–627. doi: 10.1016/0042-6822(72)90551-x. [DOI] [PubMed] [Google Scholar]
- Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ptashne M., Hopkins N. The operators controlled by the lambda phage repressor. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1282–1287. doi: 10.1073/pnas.60.4.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silva L. H., Jacob F. Etude genétique d'une mutation modifiant la sensibilité à l'immunité chez le bacteriophage lambda. Ann Inst Pasteur (Paris) 1968 Aug;115(2):145–158. [PubMed] [Google Scholar]
- Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]