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. 2012 Nov 1;86(6):1364–1375. doi: 10.1111/mmi.12061

Table 2.

Strains and plasmids used in this study

Strains Genetic characteristics Sources
AB1157 F thr1 ara14 leuB6 proA2 lacG1 tsx33 supE44 galK2 hisG4 rfbD1 mgl51 rpsL31 xyl5 mtl1 argE3 thi1 λ rac Laboratory stock
V355 F lac-3350 galK2(Oc) galT22 λ recD1014(Nuc) IN(rrnD-rrnE)1 rpsL179(strR) Shevell et al. (1988)
JW0059 F ΔpolB770::kan Δ(araD–araB)567 ΔlacZ4787(::rrnB-3) λ rph-1 Δ(rhaD–rhaB)568 hsdR514; KmR Keio Collection, NBRP
YG2004 The same as V355, but deficient in mutT with KmR cassette insertion; KmR, ΔmutT This study
YG6156 The same as AB1157, but deficient in mutT with KmR cassette insertion; KmR, ΔmutT This study
HRS7052 Deficient in a part of polA gene, thus lacking both 3′–5′ exonuclease and polymerase activities; CmR, ΔKF Wagner and Nohmi (2000)
YG6162 The same as AB1157, but deficient in dinB with in frame deletion; ΔdinB Salem et al. (2009)
YG6167 The same as YG6162, but deficient in mutT with KmR cassette insertion; KmR, ΔdinB ΔmutT This study
YG6168 The same as AB1157, but deficient in umuDC with ermGT insertion, ΔumuDC Salem et al. (2009)
YG6174 The same as YG6168, but deficient in mutT with KmR cassette insertion; KmR, ΔumuDC ΔmutT This study
YG6171 The same as YG6168, but deficient in dinB with in frame deletion, ΔumuDC ΔdinB Salem et al. (2009)
YG6172 The same as YG6171, but deficient in mutT with KmR cassette insertion; KmR, ΔumuDC ΔdinB ΔmutT This study
YG6343 The same as AB1157, but deficient in a part of polA gene, thus lacking both 3′–5′ exonuclease and polymerase activities with CmR cassette insertion; CmR, ΔKF This study
YG6347 The same as YG6343, but deficient in mutT with KmR cassette insertion; CmR; KmR, ΔKF ΔmutT This study
YG6371 The same as YG6162, but deficient in a part of polA gene, thus lacking both 3′–5′ exonuclease and polymerase activities with CmR cassette insertion; CmR, ΔdinB ΔKF This study
YG6372 The same as YG6371, but deficient in mutT with KmR cassette insertion; CmR; KmR, ΔdinB ΔKF ΔmutT This study
YG6375 The same as YG6171, but deficient in a part of polA gene with CmR cassette insertion as HRS7052 and deficient in polB gene with in frame deletion, CmR; ΔumuDC ΔdinB ΔKF ΔpolB This study
YG6376 The same as YG6375, but deficient in mutT with KmR cassette insertion; CmR; KmR, ΔumuDC ΔdinB ΔpolB ΔKF ΔmutT This study
YG6377 The same as AB1157, but deficient in polB with in frame deletion; ΔpolB This study
YG6378 The same as YG6377, but deficient in mutT with KmR cassette insertion; KmR, ΔpolB ΔmutT This study
YG6381 The same as YG6168, but deficient in polB with in frame deletion; ΔumuDC ΔpolB This study
YG6382 The same as YG6171, but deficient in polB with in frame deletion; ΔumuDC ΔdinB ΔpolB This study
YG6385 The same as YG6382, but deficient in mutT with KmR cassette insertion; KmR, ΔumuDC ΔdinB ΔpolB ΔmutT This study
YG6383 The same as YG6171, but deficient in a part of polA gene with CmR cassette insertion as HRS7052; CmR, ΔumuDC ΔdinB ΔKF This study
YG6386 The same as YG6383, but deficient in mutT with KmR cassette insertion; CmR, KmR, ΔumuDC ΔdinB ΔKF ΔmutT This study
YG6384 The same as YG6381, but deficient in a part of polA gene with CmR cassette insertion as HRS7052, CmR; ΔumuDC ΔpolB ΔKF This study
YG6387 The same as YG6384, but deficient in mutT with KmR cassette insertion; CmR; KmR, ΔumuDC ΔpolB ΔKF ΔmutT This study
YG6379 The same as YG6371, but deficient in polB with in frame deletion; CmR, ΔdinB ΔKF ΔpolB This study
YG6380 The same as YG6379, but deficient in mutT with KmR cassette insertion; CmR, KmR, ΔdinB ΔKF ΔpolB ΔmutT This study
Plasmids
pYG703 Plasmid pUC19, but has the mutT gene This study
pYG704 The same as pYG703, but has mutT::KmR This study
pCP20 pCP20 has the yeast Flp recombinase gene, FLP, chloramphenicol and ampicillin resistance genes, and temperature-sensitive replication; ApR, CmR CGSC