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Specific activities of A3G and D128K in the absence and presence of Vif variants
The specific activity was determined using the 118-nt ssDNA substrate, and the values are shown with the S.D. that was calculated from three independent experiments.
| Reaction condition | Specific activity |
|---|---|
| pmol/μg/min | |
| A3G | 10 ± 0.3 |
| A3G + VifHXB2 | 1.2 ± 0.3 |
| A3G + VifIIIB | 1.8 ± 0.9 |
| D128K | 40 ± 9 |
| D128K + VifHXB2 | 0.5 ± 0.2 |
| D128K + VifIIIB | 0.8 ± 0.4 |
