Figure 6. Nrf2 response to oxidative stress is impaired in STHdhQ111/Q111 cells.
A, An ARE reporter and a normalizing plasmid phRL-TK were transfected into striatal cells and oxidative stress was subsequently induced by treatment with tBHQ and Cu2+ for 24 h. STHdhQ7/Q7 cells show a dramatic increase in ARE activity in response to tBHQ in a dose dependent manner. However, ARE activity of STHdhQ111/Q111 cells exhibits only a mild increase at lower concentrations of tBHQ, but no further increase at higher concentrations of tBHQ. n = 3.