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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Neurobiol Dis. 2012 Dec 7;54:158–168. doi: 10.1016/j.nbd.2012.11.018

Figure 4.

Figure 4

Alterations in DA neuron morphology in response to rotenone. Confocal micrographs (60X) of nigral sections stained for TH illustrate cell morphology in an untreated group (A and B) as compared to a rotenone-treated group (E and F). Zoomed images show distinct morphological changes after rotenone administration in terms of shape, leading to elongation (C and D vs. G and H). Neuron morphologic features were measured using MetaMorph software. For cell shape analysis, a shape factor of 1 represents a circular object while a 0 value indicates a straight line (I). Although degenerating TH-immunoreactive neurons undergo changes in shape factor, quantification of area does not show any significant variations (J).