Figure 4. Rapamycin treatment prevents pathologic and behavior abnormalities in PC Tsc1 mutant mice.
A. Treatment with rapamycin (Rapa) prevented cell loss seen in vehicle (Veh) treated PC Tsc1 mutants. Scale bar: 100 μm. Quantification of cell numbers on right (n > 500cells; 2 mice per group) B., C. Whereas vehicle treated mutants displayed behavioral deficits, rapamycin treated mutants displayed amelioration of these deficits in B. reversal learning in the water T maze (n≥9 in each group) and in C. social approach in the three chambered apparatus (n≥10). As behavioral phenotypes were not significantly different between the three control genotypes in untreated mice, control genotypes were pooled into a single group for these studies. ns, p>0.05; *, p<0.05; *** p <0.001, two-way ANOVA, Bonferroni's post hoc analysis.