Table 1.
Primary antibodies
| Antigen | Immunogen | Host | Dilution | Company | Catalog No. |
|---|---|---|---|---|---|
| NPY | Synthetic NPY peptide conjugated to bovine thyroglobulin |
sheep | 1:3000 | Chemicon | AB1583 |
| NPY | Neuropeptide Y coupled to bovine thyroglobulin |
rabbit | 1:1000 | ImmunoStar | 22940 |
| CART | CART peptide 55-102 | rabbit | 1:5000 | Phoenix Pharmaceuticals |
H-003-62 |
| Orexin | carboxy terminus (amino acids 48-66) of human orexin A |
goat | 1:2000 | Santa Cruz Biotechnology |
SC-8070, C-19 |
| MCH | The full-length (19 amino acid) MCH peptide |
rabbit | 1:1000 | Phoenix Pharmaceuticals |
H-070-47 |
| GnRH | Mammalian GnRH decapeptide |
mouse | 1:5000 | Dr. Henryk Urbanski, ONPRC |
HU11B, Lot 4 |
| TPH | A recombinant rabbit TPH |
mouse | 1:1000 | Sigma | T0678, clone WH-3 |