. Author manuscript; available in PMC: 2014 May 1.

Published in final edited form as: Protein Pept Lett. 2013 May 1;20(5):573–583. doi: 10.2174/0929866511320050011

Table 3.

Thermal stability studies and secondary structure content prediction of BSA, His-Efhd2^WT and His-EFhd2 mutants at 25°C and 75°C.

	BSA		His EFhd2^WT		His EFhd2^ΔNT		His EFhd2^ΔCC
Temperature	25°C	75°C	25°C	75°C	25°C	75°C	25°C	75°C
α- helix	47.2±3.2	24.4±1.1	23.7±0.5	22.3±1.3	22.2±1.3	17.8±1.6	21.5±1.2	21.0±0.9
Antiparallel β-sheet	3.8±1.3	11.1±4.2	15.7±5.4	18.6±8.8	25.5±3.7	20.4±6.4	16.8±4.9	17.9±6.0
Parallel β-sheet	6.6±1.0	11.7±0.5	11.6±0.6	11.9±0.6	12.7±1.1	15.1±1.9	12.8±1.2	12.9±1.2
β-Turn	14.0±0.4	18.4±0.2	18.8±0.3	19.3±0.6	19.0±0.4	20.2±0.5	19.3±0.3	19.5±0.3
Random coil	29.4±4.7	40.6±1.0	39.1±1.7	39.5±1.6	42.2±1.8	47.2±2.7	42.0±2.4	42.0±2.6