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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Biochem J. 2010 May 13;428(2):255–267. doi: 10.1042/BJ20100090

Figure 5. Effects of PDGF on mitochondrial respiration.

Figure 5

Oxygen consumption rates (OCR) in VSMC: (A) VSMC were pre-treated without or with 2-deoxy-D-glucose (2-DG, 20 mM) followed by exposure to vehicle (CTRL) or PDGF (10 ng/ml) for 24 h. Baseline OCR values were first recorded followed by sequential injection of oligomycin (1 μg/ml), FCCP (1 μM) and antimycin A (10 μM). The measurements were normalized to protein following the assay. (B–D) Group data for basal OCR (panel B), maximal respiratory capacity (panel C), and mitochondrial reserve capacity (panel D), respectively. Data are presented as means ± SEM; n=4–5 per group, *p<0.05 vs. CTRL, #p<0.05 vs. PDGF.