Figure 7. L-glucose prevents PDGF-induced increases in VSMC reserve capacity and proliferation.
Oxygen consumption rates in VSMC: (A) VSMC were treated with PDGF (10 ng/ml) for 24 h in the presence of D-glucose (1 g/L) or L-glucose (1 g/L). The baseline OCR was then measured, followed by the sequential addition of oligomycin (1 μg/ml), FCCP (1 μM), and antimycin A (10 μM). The data were normalized to total protein from each well collected after the experiment. (B–D) Group data for basal OCR (panel B), maximal respiratory capacity (panel C), and mitochondrial reserve capacity (panel D), respectively. (E) Proliferation of VSMC in D- or L-glucose-containing media after treatment with vehicle (CTRL) or PDGF (10 ng/ml) for 48 h. (F) Glycolytic flux in VSMC cultured in D- or L-glucose-containing media exposed to vehicle (CTRL) or PDGF for 24 h. Data are presented as means ± SEM; n=3–5 per group, *p<0.05 vs. CTRL, #p<0.05 vs. PDGF.