Table II.
Used primers and hydrolysis probes in this study.
| Sequence (5′→3′) | |
|---|---|
| For PCR analysis | |
| ABCB6 | |
| 5′-primer | GGACCAAGATGTGGAAAGGA |
| 3′-primer | CCAAAATCTCGCCAGGTAGA |
| Hydrolysis probe | UPL probe no. 66a |
| GAPDH | |
| 5′-primer | AGCCACATCGCTCAGACAC |
| 3′-primer | GCCCAATACGACCAAATCC |
| Hydrolysis probe | UPL probe no. 60a |
| ACTB | |
| 5′-primer | CCAACCGCGAGAAGATGA |
| 3′-primer | CCAGAGGCGTACAGGGATAG |
| Hydrolysis probe | UPL probe no. 64a |
| For qMSP analysisb | |
| ABCB6-MSP1 (position: −327 to −207) | |
| 5′-primer | GGGGTTATAGTcgTGGAGc |
| 3′-primer | AAAACAcgTAcgCcgTCT |
| Hydrolysis probe | FAM-GTGGGTTTGTAGTTGGTAGGAGGGTT-BHQ |
| ABCB6-MSP2 (position: −710 to −607) | |
| 5′-primer | TAGATTTTTTGTTGTTTcgc |
| 3′-primer | TCTAAAcgAcgACCTAAACA |
| Hydrolysis probe | FAM-AAGAGAAATGGGATGGGGATTTTG-BHQ |
| RPPH1 | |
| 5′-primer | AATGAGGTGTAGAAGGTTGATGGT |
| 3′-primer | CATAATTAAATCACTTCCCACCAAA |
| Hydrolysis probe | UPL Probe no. 10a |
a
Universal ProbeLibrary (Roche Applied Bioscience).
b
CpG sites are presented as lower case letters. Position was given relative to the first codon of ABCB6. Start site of the first exon of ABCB6 is positioned at −317.