Figure 2. Tetracycline antibiotics affect glucose metabolism and oxygen consumption in a dose-dependent fashion.
Effects of the tetracyclines on glycolytic flux were determined by measuring changes in A) glucose consumption and B) lactate production rates of MCF12A cells when treated with Dox, Mino, or Tet at 100 ng/mL or 1 µg/mL. A dose-response relationship was established between Dox and C) lactate production rate in MCF12A cells, D) lactate production rate in 293T cells, E) oxygen consumption rate in MCF12A cells, and F) oxygen consumption rate in 293T cells by treating with a range of drug concentrations. Error bars represent standard deviation from experimental triplicate measurements for all assays, except for oxygen consumption dose-response in 293T cells, which was performed with 8 replicates. * denotes a p-value ≤0.05, ** a p-value ≤0.01 from a two-tailed Student’s t-test.