Fig. 2.
RSV proteins were detected in lung and brain of infected mice. Confocal photomicrograph sections of lung and brain tissue. Viral proteins were observed by immunofluorescence (green fluorescence) in a confocal microscope using an anti-F-RSV or anti–N-RSV antibody. The nuclei were stained with thiazole orange-oligonucleotide conjugates (TOPRO-3) (blue). (A) Images of infected lung tissues 3 d after RSV infection. (Upper) Staining for F protein. (Lower) Staining for N protein. (B) Pictures of infected brain cortex 3 d after RSV infection. (Upper) Staining for F protein. (Lower) Staining for RSV Nucleoprotein. (C) Images of infected olfactory bulb tissues 1 d after RSV infection. (Upper) Staining for the F protein. (Lower) Staining for the nucleoprotein. (D) Images of infected choroid plexus after 3 d of RSV infection. (Upper) Staining for F protein. (Lower) Staining for N protein. (Scale bar, 150 µm.) (E) Quantification of total F-RSV channel fluorescence intensity per 40× field by pixel analysis of immunofluorescence for whole-brain and lung. Mouse α-F RSV, primary antibody; goat α-mouse, secondary antibody. More than 10 fields were analyzed per treatment.