Table 1.

Optimization of the qNPA protocol.

Solutions/steps	Incubation temp	Original time	Optimized time
Denaturation	95°C	20 min	5 min
Hybridization	60°C	6 hours	2 hours
S1 nuclease reaction	50°C	90 min	60 min
Stop reaction	95°C	15 min	15 min
Cool plate	RT	10 min	5 min
Transfer to array	50°C	16 hours	4 hours
Detection linker	60°C	90 min	60 min
Detection enzyme	37°C	60 min	30 min
Protocol time		27 hours	9 hours