Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1982 Apr;2(4):339–345. doi: 10.1128/mcb.2.4.339

Cells of pea (Pisum sativum) that differentiate from G2 phase have extrachromosomal DNA.

J Van't Hof, C A Bjerknes
PMCID: PMC369797  PMID: 7110135

Abstract

Velocity sedimentation in an alkaline sucrose gradient of newly replicated chromosomal DNA revealed the presence of extrachromosomal DNA that was not replicated by differentiating cells in the elongation zone. The extrachromosomal DNA had a number average molecular weight of 12 X 10(6) to 15 X 10(6) and a weight average molecular weight of 25 X 10(6), corresponding to about 26 X 10(6) and 50 X 10(6) daltons, respectively, of double-stranded DNA. The molecules were stable, lasting at least 72 h after being formed. Concurrent measurements by velocity sedimentation, autoradiography, and cytophotometry of isolated nuclei indicated that the extrachromosomal molecules were associated with root-tip cells that stopped dividing and differentiated from G2 phase but not with those that stopped dividing and differentiated from G1 phase.

Full text

PDF
339

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun R., Evans T. E. Replication of nuclear satellite and mitochondrial DNA in the mitotic cycle of Physarum. Biochim Biophys Acta. 1969 Jun 17;182(2):511–522. doi: 10.1016/0005-2787(69)90203-2. [DOI] [PubMed] [Google Scholar]
  2. Carrier W. L., Setlow R. B. Paper strip method for assaying gradient fractions containing radioactive macromolecules. Anal Biochem. 1971 Oct;43(2):427–432. doi: 10.1016/0003-2697(71)90272-7. [DOI] [PubMed] [Google Scholar]
  3. DEELEY E. M., DAVIES H. G., CHAYEN J. The DNA content of cells in the root of Vicia faba. Exp Cell Res. 1957 Jun;12(3):582–591. doi: 10.1016/0014-4827(57)90174-x. [DOI] [PubMed] [Google Scholar]
  4. Fox D. P. Some characteristics of the cold hydrolysis technique for staining plant tissues by the feulgen reaction. J Histochem Cytochem. 1969 Apr;17(4):266–272. doi: 10.1177/17.4.266. [DOI] [PubMed] [Google Scholar]
  5. Gall J. G. Free ribosomal RNA genes in the macronucleus of Tetrahymena. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3078–3081. doi: 10.1073/pnas.71.8.3078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  7. Kolodner R. D., Tewari K. K. Denaturation mapping studies on the circular chloroplast deoxyribonucleic acid from pea leaves. J Biol Chem. 1975 Jul 10;250(13):4888–4895. [PubMed] [Google Scholar]
  8. Kolodner R., Tewari K. K. Physicochemical characterization of mitochondrial DNA from pea leaves. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1830–1834. doi: 10.1073/pnas.69.7.1830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kudirka D. T., Van't Hof J. Increased resolution of cytophotometric measurements by mean extinction and area parameters of Tradescantia nuclei. Anal Quant Cytol. 1980 Jun;2(2):99–106. [PubMed] [Google Scholar]
  10. Murray M. G., Thompson W. F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980 Oct 10;8(19):4321–4325. doi: 10.1093/nar/8.19.4321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schvartzman J. B., Chenet B., Bjerknes C., Van't Hof J. Nascent replicons are synchronously joined at the end of S phase or during G2 phase in peas. Biochim Biophys Acta. 1981 Apr 27;653(2):185–192. doi: 10.1016/0005-2787(81)90154-4. [DOI] [PubMed] [Google Scholar]
  12. Van't Hof J. Cell population kinetics of excised roots of Pisum sativum. J Cell Biol. 1965 Oct;27(1):179–189. doi: 10.1083/jcb.27.1.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Van't Hof J. PEA (Pisum sativum) cells arrested in G2 have nascent DNA with breaks between replicons and replication clusters. Exp Cell Res. 1980 Sep;129(1):231–237. doi: 10.1016/0014-4827(80)90346-8. [DOI] [PubMed] [Google Scholar]
  14. Van't Hof J. Studies on the relationships between cell population and growth kinetics of root meristems. Exp Cell Res. 1967 May;46(2):335–347. doi: 10.1016/0014-4827(67)90071-7. [DOI] [PubMed] [Google Scholar]
  15. Vogt V. M., Braun R. The replication of ribosomal DNA in Physarum polycephalum. Eur J Biochem. 1977 Nov 1;80(2):557–566. doi: 10.1111/j.1432-1033.1977.tb11912.x. [DOI] [PubMed] [Google Scholar]
  16. Ward B. L., Anderson R. S., Bendich A. J. The mitochondrial genome is large and variable in a family of plants (cucurbitaceae). Cell. 1981 Sep;25(3):793–803. doi: 10.1016/0092-8674(81)90187-2. [DOI] [PubMed] [Google Scholar]
  17. van Oostveldt P., van Parijs R. Underreplication of repetitive DNA in polyploid cells of Pisum sativum. Exp Cell Res. 1976 Mar 1;98(1):210–221. doi: 10.1016/0014-4827(76)90481-x. [DOI] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES