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. Author manuscript; available in PMC: 2013 Jul 11.
Published in final edited form as: Nat Biotechnol. 2012 Jan 15;30(2):159–164. doi: 10.1038/nbt.2106

Figure 3.

Figure 3

Analysis of pleiotropy and locus heterogeneity. (a) Fraction of mutation pairs on the same protein causing different diseases. **P < 10−20. P-values calculated using binomial tests. (b) Illustration of WASP and its interaction interfaces with CDC42 and VASP. Colored stars indicate locations of experimentally tested mutations. (c) Effects on the WASP-CDC42 interaction by mutations on different interaction interfaces tested by Y2H. Flag tagged wild-type and mutant WASP were expressed in HEK293T cells, western blot analysis showed similar levels of WASP proteins. γ -tubulin was used as a loading control. (d) Fraction of mutation pairs on two proteins causing the same disease.