Figure 4.
AMPK is required for cytotoxic T lymphocytes (CTLs) adaptation to metabolic stress in vivo but dispensable for lymphopenia-induced proliferation. (A) Control (gray shade) and AMPKα1null (thick line) polyclonal CTLs used for adoptive cotransfer gated on CD45.1 congenic marker, expression of CD62L and CD44. (B) Frequency of cotransferred CFSEhi CD8 CD45 congenically marked CTLs 7 days after adoptive transfer. Each symbol represents recovered cells from an individual Ly5.1 recipient among CD8 cells, LN left panel, spleen middle panel or Rag2-/- recipient spleen among leukocytes, right panel. Data shown are pooled data from three experiments, paired t-test. (C) Frequency of recovered control and AMPKα1null naïve CD8 T cells co-injected into Rag2-/- mice at day 14 after adoptive transfer. Data shown are pooled from two experiments, each symbol represents recovered CD8 cells from an individual recipient.