Fig. 1.
Distribution of Met mRNA in the developing mouse brainstem. Series of coronal sections through the caudo-rostral extent of E13 (a, b, c), E15 (d, e, f), and E17 (g, h, i) brainstem hybridized with DIG-labeled riboprobe to the MET receptor tyrosine kinase. Arrows point to areas shown at higher magnification (boxed). Dorsal (D) is to the top. DMV, dorsal nucleus of the tenth nerve (motor, vagus); nA, ambiguous nucleus; nTS, nucleus of the solitary tract; DR, dorsal raphe nuclei; BN, Barrington’s nucleus; Aq, Aqueduct; nVII, nucleus of the seventh nerve; nXII, nucleus of the twelfth nerve (hypoglossal). Met expression was also detected in the superior colliculus (asterisk). The anatomical labeling of brainstem structures were determined by comparing the expression patterns on serial sections from several experiments with Chemoarchitectionic Atlas of the Developing Mouse Brain (Jacobowitz and Abbott 1997) and with Isl1 expression on adjacent sections at E13 (supplemental fig. 1). Scale bars=30 μm. Insert: a-d and g, magnified 3 times; e, f, h, and i, magnified 4 times.