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. Author manuscript; available in PMC: 2013 Oct 12.
Published in final edited form as: Cell. 2012 Oct 12;151(2):400–413. doi: 10.1016/j.cell.2012.09.010

Figure 2. UCP1 Is Activated by Endogenous Membrane LCFAs.

Figure 2

(A) Left panel: Representative time course of IUCP1 amplitude in control (1), upon application of 0.5% BSA (2), and with 0.5% BSA and 1 mM GDP (3). AA (5 μM) was applied at the end to verify that IUCP1 can still be activated (4). Pipette solution contained 0.5% BSA. Amplitudes were measured upon stepping from 0 to –160 mV (see right panel). Right panel: IUCP1 traces recorded at times 1, 2, 3, and 4 as indicated in the left panel.

(B) The same experiment as in (A) but performed with 15 mM αCD in the bath and pipette solutions.

(C) Left panel: Representative time course of IUCP1 amplitude in control (1) and upon the application (2) and subsequent washout (3) of 10 mM αCD at pH 8.0. Right panel: IUCP1 traces recorded at times 1, 2, and 3 as indicated in the left panel.

(D) The same experiment as in (C) but performed at symmetrical pH 6.0.

See also Figure S2.