Figure 2.
Mitochondrial fragmentation in living APP/PS1 transgenic mouse brain. A, Examples of lenti-mtGFP-labeled mitochondrial structure in neurons in a Wt and APP/PS1transgenic mouse. In the top images, neurons in the brain of a Wt mouse exhibit normal mitochondrial morphology. The bottom images show a neuron near plaques in a Tg mouse brain that shows severe fragmentation of mitochondria as a result of excessive mitochondrial fission. Scale bar, 40 μm. B, Frequency distribution of mitochondrial length from neurons in A. Red, neurons with mitochondrial fragmentation; black, neurons with normal mitochondria. C, Results of counting the number of neurons with mitochondrial fragmentation per volume in Tg versus Wt mice (n = 6 mice per genotype).