TABLE 1.
Mean rates ± S.D.s of formation of MFA conjugates incubated with Gly, Tau, GSH, and NAC (10 mM) in physiologic buffer (pH 7.4, 37°C) (n = 3)
Conjugate | MFA-Gly | MFA-Tau | MFA-GSH | MFA-NAC |
---|---|---|---|---|
1 μM | nM/min | |||
MFA-AMP | 0.39 ± 0.07 | 0.35 ± 0.01 | 0.21 ± 0.02 | 0.12 ± 0.08 |
MFA-CoA | N.D. | 0.02 ± 0.01 | 2.42 ± 0.67 | 2.34 ± 0.08 |
MFA-GSH | N.A. | N.A. | N.A. | 13.0 ± 0.44 |
MFA-1-O-G | N.D. | N.D. | N.D. | N.D. |
N.A., not applicable; N.D., not detected.