TABLE 1.
Genes regulated by coumermycin in GyrB.PKR-expressing cells
| Gene | Function | Regulation (fold) by coumermycin in cell line expressing:
|
Reference(s) | |||
|---|---|---|---|---|---|---|
| GyrB.PKR | GyrB.PKRK296H | GyrB.PKR + FLAG-HPV-18 E6 | GyrB.PKR + FLAG-HPV-11 E6 | |||
| Human homologue of yeast YA22 (HYA22) | Putative tumor suppressor | −29.3 | −1.5 | −1.9 | −10.1 | 31, 60 |
| Human homologue of rat pituitary tumor-transforming gene (PTTG3) | Proto-oncogene, regulator of cell proliferation and survival | −20.0 | 1.2 | −2.2 | −1.9 | 30, 63 |
| Cyclin E2 (CCNE2) | Cell cycle, overexpressed in human cancers | −13.5 | −1.4 | −2.5 | −2.6 | 58 |
| Radiation sensitivity 54L (RAD54L) | Double-stranded DNA-dependent ATPase, DNA repair, putative tumor suppressor | −12.8 | −1.8 | −1.2 | −3.3 | 44 |
| Rho GDP dissociation inhibitor (GDI) beta (ARHGDIB) | Extracellular signaling, cyto-skeleton organization, cell mobility | −12.0 | 1.0 | −1.3 | −3.4 | 56 |
| Regulator of G-protein signaling 4 (RGS4) | Regulation of G-protein function | −6.6 | 1.3 | −1.7 | −1.2 | 52 |
| Cyclin E1 (CCNE1) | Cell cycle, G/S transition | −5.6 | −2.0 | −1.4 | −1.3 | 58 |
| Peroxiredoxin 1 (PRDX1) | Redox regulation, cell signaling, and apoptosis | −5.3 | −1.0 | −1.1 | −1.9 | 82 |
| Centromere-associated protein E (CENPE) | Spindle microtubule organization, mitotic checkpoint signaling | −5.1 | 1.0 | −1.1 | −1.8 | 83 |
| Lymphotoxin beta (LT-beta; tumor necrosis factor superfamily, member 3) | Apoptosis, inflammatory responses | 5.4 | 1.4 | −1.9 | −1.4 | 79 |
| RNA polymerase III subunit 62 (RPC62) | Transcription of short mRNAs, involved in tRNA splicing | 5.5 | 1.4 | 1.4 | 2.9 | 70 |
| Methylthioadenosine phosphorylase (MTAP) | Putative tumor suppressor | 5.6 | −1.4 | −1.1 | 13.6 | 11, 18 |
| UV radiation resistance-associated gene (UVRAG) | Complement to UV sensitivity of xeroderma pigmentosum complementation group C | 5.7 | 1.1 | 1.1 | 1.5 | 59 |
| Protein kinase AMP-activated, beta-1 noncatalytic subunit (PRKAB1) | Adaptation to metabolic stress | 5.8 | −1.3 | 1.5 | 2.2 | 80 |
| Keratin 7 (KRT7) | Marker of epithelial differentiation | 6.2 | −1.1 | 1.6 | 1.9 | 40 |
| Thioredoxin reductase (TR) | Cellular defense against oxygen damage | 6.3 | 1.6 | −1.1 | 1.5 | 29 |
| Melanoma differentiation-associated gene 7 (mda-7)/interleukin 24 (IL-24) | Tumor suppressor | 6.4 | 1.2 | −2.7 | 4.1 | 68 |
| Retinoic acid receptor alpha (RARα) | Disruption of tumor progression | 7.0 | 1.1 | 1.1 | 1.7 | 33 |
| IRF1 | Transcription factor, cell cycle and apoptosis | 7.8 | 1.2 | 1.2 | 2.6 | 77 |
| Transcription factor RelB (RELB) | Regulation of development, cell cycle, cell survival | 8.0 | 1.4 | 1.5 | 1.6 | 22 |
| Growth arrest and DNA damage inducible 45A (GADD45A) | Regulator of DNA damage, apoptosis | 8.4 | 1.5 | 2.7 | 2.6 | 72 |
| Growth arrest and DNA damage gene 34 (GADD34) | Promoter of eIF2α dephosphorylation | 9.7 | 1.9 | 4.7 | 10.8 | 54, 55 |
| Growth arrest and DNA damage inducible 45B (GADD45B) | Regulator of DNA damage, apoptosis | 10.2 | 1.5 | −2.1 | 7.3 | 72 |
| Transcription factor JunB (JUNB) | Regulation of cell differentiation, proliferation, and apoptosis | 10.5 | 2.0 | 1.5 | 1.4 | 71 |
| Tumor necrosis factor alpha receptor-associated factor 1 (TRAF1) | Adaptor protein, negative regulator of tumor necrosis factor signaling | 11.9 | 1.2 | 1.7 | 2.6 | 85 |
| BH3-only Bcl-2 homologous protein BIK | Inducer of apoptosis | 13.0 | −1.4 | 6.6 | −1.1 | 61 |
| Tumor necrosis factor alpha-induced protein 3 (TNFAIP3) or A20 | Transcription-growth regulatory factor, involved in NF-κB pathway | 15.9 | 2.0 | −1.3 | 1.9 | 5 |
| Winged helix/forkhead transcription factor (FOXO3A) | Transcription factor, cell cycle, apoptosis, cell metabolism, and oxidative stress | 23.0 | −1.2 | 1.8 | 6.2 | 7 |
| IFN stimulatory gene 20 (ISG20) | IFN-induced RNase, 3′ to 5′ exonuclease, antiviral activity | 26.9 | 1.1 | 3.3 | 3.6 | 20 |
| Vitamin D3-upregulated protein (VDUP1/TXNIP) | Major regulator of cellular redox state, inhibitor of thioredoxin and tumor metastasis | 27.8 | 1.7 | 3.7 | 3.5 | 84 |
| Early growth response 1 (EGR1) transcription factor | Regulator of tumorigenesis | 42.8 | 2.8 | 2.0 | 5.2 | 1 |