Table 1.
Summary of sarkosyl-insoluble and immunohistochemical analysis of tau measured from female and male TauP301L and LRRK2/TauP301L mice
| Stain | Genotype | Sex | Interaction | |||
|---|---|---|---|---|---|---|
| F(DFn, DFd) | P value | F(DFn, DFd) | P value | F(DFn, DFd) | P value | |
| Two-way ANOVA of insoluble pTau normalized to insoluble human tau | ||||||
| pT149 | F(1, 14) = 15 | P < 0.005 | F(1, 14) = 0.001 | P = 0.97 | F(1, 14) = 0.01 | P = 0.92 |
| pT153 | F(1, 14) = 3.8 | P = 0.07 | F(1, 14) = 1.0 | P = 0.33 | F(1, 14) = 0.16 | P = 0.70 |
| AT270 | F(1, 14) = 7.73 | P ≤ 0.05 | F(1, 14) = 33.44 | P < 0.0001 | F(1, 14) = 0.66 | P = 0.43 |
| CP13 | F(1, 14) = 0.25 | P = 0.62 | F(1, 14) = 1.59 | P = 0.23 | F(1, 14) = 4.47 | P ≤ 0.05 |
| pT205 | F(1, 14) = 5.4 | P ≤ 0.05 | F(1, 14) = 1.9 | P = 0.19 | F(1, 14) = 0.01 | P = 0.92 |
| AT8 | F(1, 14) = 24 | P < 0.0005 | F(1, 14) = 64 | P < 0.0001 | F(1, 14) = 8.5 | P ≤ 0.05 |
| pT212 | F(1, 14) = 1.05 | P = 0.32 | F(1, 14) = 0.03 | P = 0.87 | F(1, 14) = 0.12 | P = 0.74 |
| pS214 | F(1, 14) = 0.02 | P = 0.90 | F(1, 14) = 0.07 | P = 0.79 | F(1, 14) = 2.17 | P = 0.16 |
| AT100 | F(1, 14) = 0.25 | P = 0.63 | F(1, 14) = 1.49 | P = 0.24 | F(1, 14) = 4.89 | P = 0.04 |
| PHF1 | F(1, 14) = 0.76 | P = 0.40 | F(1, 14) = 11.63 | P < 0.005 | F(1, 14) = 4.14 | P = 0.06 |
| TauP301L = 4 females, 5 males; LRRK2/TauP301L =5 females, 4 males | ||||||
| Two-way ANOVA of Tau Cortical Burden | ||||||
| pT149 | F(1, 14) = 4.5 | P ≤ 0.05 | F(1, 14) = 13 | P < 0.005 | F(1, 14) = 0.14 | P = 0.71 |
| pT153 | F(1, 14) = 5.0 | P ≤ 0.05 | F(1, 14) = 14 | P < 0.005 | F(1, 14) = 0.53 | P = 0.48 |
| AT270 | F(1, 14) = 0.22 | P = 0.65 | F(1, 14) = 0.24 | P = 0.63 | F(1, 14) = 0.32 | P = 0.58 |
| CP13 | F(1, 14) = 4.6 | P ≤ 0.05 | F(1, 14) = 6.0 | P ≤ 0.05 | F(1, 14) = 0.17 | P = 0.69 |
| AT8 | F(1, 14) = 8.5 | P ≤ 0.05 | F(1, 14) = 16 | P < 0.005 | F(1, 14) = 2.1 | P = 0.17 |
| MC1 | F(1, 14) = 6.1 | P ≤ 0.05 | F(1, 14) = 8.8 | P ≤ 0.05 | F(1, 14) = 0.76 | P = 0.40 |
| TauP301L = 5 females, 4 males; LRRK2/TauP301L = 5 females, 4 males | ||||||
Top statistical results from a two-way ANOVA (genotype × sex) of the densitometry of phospho-specific tau antibodies normalized to the amount of human tau in the insoluble fraction for each mouse. Bottom Statistical results from a two-way ANOVA (genotype × sex) of cortical staining with phospho- and conformational-specific tau antibodies