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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Immunol. 2013 Aug 9;191(6):10.4049/jimmunol.1301163. doi: 10.4049/jimmunol.1301163

Figure 6. Changes to the BM cellular compartments following Salmonella infection or bacterial adjuvant injection.

Figure 6

(A) Frequencies of CD19+ (B cells) and Gr1+ cells (granulocytes) in the BM of naïve or Salmonella infected mice (at day 16). (B) Frequency of eosinophils (siglec-Fhi / CD11bint) in the bone of naïve or Salmonella infected mice (at day 16). Plots from (A) and (B) are representative of 5 mice from 1 of 5 experiments. (C) CXCL12 levels in the supernatant of BM cultures from mice Salmonella infected (at day 16). (D) CXCL12 levels in the supernatant of BM cultures from mice receiving no boost, soluble FITC-OVA boost, or FITC-OVA + adjuvant boost at day 5, 12 and 60 (all mice primed with alum FITC-OVA + B pertussis). Each point is data from 1 mouse, bars represent the mean, this is representative of 3 other experiments. Migration across transwells towards medium or medium containing CXCL12 (400nM) or CXCL9 (400nM); expressed as the percentage of FITC-OVA IgG PC that migrated across the transwell). Mean and standard errors of ELISA titers from 5 mice per group (experiment run 3 times). Statistical significance was evaluated using student’s t test (C) or one-way ANOVA with Bonferroni post test (D).