Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Jun;76(6):2679–2683. doi: 10.1073/pnas.76.6.2679

Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA.

A Dasgupta, M H Baron, D Baltimore
PMCID: PMC383671  PMID: 223155

Abstract

The soluble phase of the cytoplasm of poliovirus-infected cells contains an enzymatic activity able to copy RNA without an added primer. This replicase activity has been purified 60-fold; it is absent from uninfected cells. Poly(U) polymerase activity copurifies with replicase activity. Although less pure replicase fractions copy a variety of RNAs, purer fractions respond better to poliovirus RNA than to other viral RNAs. Even the less pure fractions make a specific copy of the added template, as shown by hybridization of the product to its template RNA but not to other RNAs. Among homopolymers only poly(A)-oligo(U) was copied by the replicase; other primed homopolymer templates were inactive.

Full text

PDF
2679

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALTIMORE D., EGGERS H. J., FRANKLIN R. M., TAMM I. Poliovirus-induced RNA polymerase and the effects of virus-specific inhibitors on its production. Proc Natl Acad Sci U S A. 1963 Jun;49:843–849. doi: 10.1073/pnas.49.6.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D., Girard M., Darnell J. E. Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology. 1966 Jun;29(2):179–189. doi: 10.1016/0042-6822(66)90024-9. [DOI] [PubMed] [Google Scholar]
  3. Bowles S. A., Tershak D. R. Proteolysis of noncapsid protein 2 of type 3 poliovirus at the restrictive temperature: breakdown of noncapsid protein 2 correlates with loss of RNA synthesis. J Virol. 1978 Aug;27(2):443–448. doi: 10.1128/jvi.27.2.443-448.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  6. Caliguiri L. A., Tamm I. The role of cytoplasmic membranes in poliovirus biosynthesis. Virology. 1970 Sep;42(1):100–111. doi: 10.1016/0042-6822(70)90242-4. [DOI] [PubMed] [Google Scholar]
  7. De Wachter R., Fiers W. Sequences at the 5'-terminus of bacteriophage Q-beta-RNA. Nature. 1969 Jan 18;221(5177):233–235. doi: 10.1038/221233a0. [DOI] [PubMed] [Google Scholar]
  8. Flanegan J. B., Baltimore D. Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A). Proc Natl Acad Sci U S A. 1977 Sep;74(9):3677–3680. doi: 10.1073/pnas.74.9.3677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haruna I., Spiegelman S. Recognition of size and sequence by an RNA replicase. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1189–1193. doi: 10.1073/pnas.54.4.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lundquist R. E., Maizel J. V., Jr In vivo regulation of the poliovirus RNA polymerase. Virology. 1978 Sep;89(2):484–493. doi: 10.1016/0042-6822(78)90190-3. [DOI] [PubMed] [Google Scholar]
  11. Nomoto A., Detjen B., Pozzatti R., Wimmer E. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis. Nature. 1977 Jul 21;268(5617):208–213. doi: 10.1038/268208a0. [DOI] [PubMed] [Google Scholar]
  12. Pettersson R. F., Flanegan J. B., Rose J. K., Baltimore D. 5'-Terminal nucleotide sequences of polio virus polyribosomal RNA and virion RNA are identical. Nature. 1977 Jul 21;268(5617):270–272. doi: 10.1038/268270a0. [DOI] [PubMed] [Google Scholar]
  13. Rosenberg H., Diskin B., Oron L., Traub A. Isolation and subunit structure of polycytidylate-dependent RNA polymerase of encephalomyocarditis virus. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3815–3819. doi: 10.1073/pnas.69.12.3815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spector D. H., Baltimore D. Polyadenylic acid on poliovirus RNA. II. poly(A) on intracellular RNAs. J Virol. 1975 Jun;15(6):1418–1431. doi: 10.1128/jvi.15.6.1418-1431.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stanley J., Rottier P., Davies J. W., Zabel P., Van Kammen A. A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Res. 1978 Dec;5(12):4505–4522. doi: 10.1093/nar/5.12.4505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Traub A., Duskin B., Rosenberg H., Kalmar E. Isolation and properties of the replicase of encephalomyocarditis virus. J Virol. 1976 May;18(2):375–382. doi: 10.1128/jvi.18.2.375-382.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Young N. A., Hoyer B. H., Martin M. A. Polynucleotide sequence homologies among polioviruses. Proc Natl Acad Sci U S A. 1968 Oct;61(2):548–555. doi: 10.1073/pnas.61.2.548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Zabel P., Jongen-Neven I., van Kammen A. In Vitro Replication of Cowpea Mosaic Virus RNA III. Template Recognition by Cowpea Mosaic Virus RNA Replicase. J Virol. 1979 Jan;29(1):21–33. doi: 10.1128/jvi.29.1.21-33.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. el-Manna M. M., Bruening G. Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. Virology. 1973 Nov;56(1):198–206. doi: 10.1016/0042-6822(73)90299-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES