Table II.
Effects of MAP kinase inhibitors on LPS- and E.coli K-12-induced IL-8 secretion in CD36-overexpressing HEK 293 cells a
| MAPK Inhibitor b | IL-8 (ng/mg cell protein) | |
|---|---|---|
| LPS | E.coli K-12 | |
| PD 98059 (MEK inhibitor – MAP kinase kinase | ||
| 0 µM | 3.91 ± 0.35 | 4.27 ± 0.41 |
| 5 µM | 3.72 ± 0.09 | 4.28 ± 0.24 |
| 20 µM | 3.46 ± 0.13 | 3.62 ± 0.14 |
| 50 µM | 3.43 ± 0.21 | 3.86 ± 0.17 |
| SB 203589 (p38 MAPK Inhibitor) | ||
| 0 µM | 3.91 ± 0.35 | 4.27 ± 0.41 |
| 1 µM | N/D | 4.51 ± 0.19 |
| 5 µM | 4.06 ± 0.14 | 5.49 ± 0.48 |
| 25 µM | 5.21 ± 0.31 | 6.02 ± 0.41 |
| SP 600125 (JNK 1/2/3 Inhibitor) | ||
| 0 µM | 3.91 ± 0.35 | 4.27 ± 0.41 |
| 5 µM | 2.07 ± 0.14 c | 2.09 ± 0.08 c |
| 20 µM | 1.81 ± 0.16 c | 1.59 ± 0.21 c |
| 50 µM | N/D | 1.36 ± 0.49 c |
| JNK Inhibitor JNKi-III | ||
| 0 µM | 3.91 ± 0.35 | 4.27 ± 0.41 |
| 5 µM | 3.27 ± 0.15 | 2.99 ± 0.17 c |
| 25 µM | 1.66 ± 0.12 c | 1.41 ± 0.15 c |
| Negative Control for JNK Inhibitor JNKi-III | ||
| 0 µM | 3.91 ± 0.35 | 4.27 ± 0.41 |
| 5 µM | 3.62 ± 0.21 | 4.33 ± 0.23 |
| 25 µM | 3.26 ± 0.36 | 3.61 ± 0.39 |
a
Data are presented as means ± S.D. of one of two separate experiments that were performed in duplicates and yielded similar results.
b
Cells were treated with the indicated concentrations of inhibitors for 1hr prior to a 20h stimulation with LPS (100ng/ml) or E. coli K-12 (2ng/ml).
c
p < 0.05, IL-8 levels in the presence of inhibitor vs. control (in the absence of inhibitor), Student’s t test.