Figure 5.
BRP-39 deletion inhibits M2 phenotype in macrophages. Attenuated expression of M2 marker genes Arg1 and Fizz1 in BRP-39−/− peritoneal macrophages stimulated with interleukin-4 (IL-4; 10 ng/mL) for 16 h ((a) and (b)). Protein levels (Western blot and densitometry) of M2 markers Arg1 and Ym1 in bone marrow derived macrophages (BMDMs) (c). Results expressed as the mean ± SEM of data obtained from three independent experiments stimulated with IL-4 (10 ng/mL) for 24 h. C: control (RA); HYP: hyperoxia; BRP39 KO: BRP39 knock out or BRP-39−/−. *P < 0.05, **P < 0.01, and ***P < 0.001.