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. 1974 Jun;71(6):2372–2376. doi: 10.1073/pnas.71.6.2372

Activity of Empty, Headlike Particles for Packaging of DNA of Bacteriophage λ In Vitro

B Hohn 1, T Hohn 1
PMCID: PMC388457  PMID: 4601587

Abstract

A precursor head of phage lambda is synthesized after induction of cells lysogenic for λD-F- and λA- (head-defective) mutants. This precursor head can be assayed by complementation in vitro and can be purified by CsCl gradient centrifugation and sucrose gradient sedimentation. The precursor head contains no DNA and has the same dimensions as the petit λ particle. It can be packed with λ DNA in an extract from induced Escherichia coli lysogenic for a λE- mutant.

Keywords: morphogenesis, DNA condensation, in vitro complementation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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