Figure 5.
Treatment with UA-8 preserves a healthy pool of mitochondria during starvation. Activities of key mitochondrial enzymes were assessed in HL-1 cells and NCMs following 24 h of starvation. Citrate synthase (a, d), succinate dehydrogenase (b, e) and COX IV (c, f) activities were measured in HL-1 cells and NCMs in nonstarved (NS) and starved cells (24 h STV) treated with UA-8 (1 μM) or without 14,15-EEZE (10 μM). Increased expression of mitochondrial proteins (g) VDAC, (h) succinate dehydrogenase and (i) COX IV in NCMs following 24 h of starvation were observed in both control and UA-8-treated cells, as detected by western blot. Values are represented as mean±S.E.M., N=3. Significance was P<0.05, *significantly different from control nonstarvation, #significantly different from UA-8