Abstract
Seventeen hydrophilic proteins and five amphiphilic membrane proteins were subjected to agarose gel electrophoresis in the presence of a nonionic detergent (Triton X-100), a mixture of anonionic and an anionic detergent (Triton X-100 and sodium deoxycholate), and a mixture of a nonionic and a cationic detergent (Triton X-100 and cetyltrimethylammonium bromide). The electrophoretic mobility of the hydrophilic proteins was unaffected in the three detergent mixtures. However, the mobility of the amphiphilic proteins shifted anodally in the Triton X-100-deoxycholate system and cathodally in the Triton X-100-cetyltrimethylammonium bromide system when compared to the mobility in Triton X-100 alone. The detergent-induced shift in mobility provides a simple, rapid, and sensitive method for distinguishing between hydrophilic and amphiphilic proteins.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Borgström B., Donnér J. Binding of bile salts to pancreatic colipase and lipase. J Lipid Res. 1975 Jul;16(4):287–292. [PubMed] [Google Scholar]
- Charles M., Sari H., Entressangles B., Desmuelle P. Interaction of pancreatic colipase with a bile salt micelle. Biochem Biophys Res Commun. 1975 Jul 22;65(2):740–745. doi: 10.1016/s0006-291x(75)80207-5. [DOI] [PubMed] [Google Scholar]
- Clarke S. The size and detergent binding of membrane proteins. J Biol Chem. 1975 Jul 25;250(14):5459–5469. [PubMed] [Google Scholar]
- Fries E. Determination of Triton X-100 binding to membrane proteins by polyacrylamide gel electrophoresis. Biochim Biophys Acta. 1976 Dec 14;455(3):928–936. doi: 10.1016/0005-2736(76)90061-4. [DOI] [PubMed] [Google Scholar]
- Helenius A., Fries E., Garoff H., Simons K. Solubilization of the Semliki Forest virus membrane with sodium deoxycholate. Biochim Biophys Acta. 1976 Jun 17;436(2):319–334. doi: 10.1016/0005-2736(76)90197-8. [DOI] [PubMed] [Google Scholar]
- Helenius A., Simons K. Solubilization of membranes by detergents. Biochim Biophys Acta. 1975 Mar 25;415(1):29–79. doi: 10.1016/0304-4157(75)90016-7. [DOI] [PubMed] [Google Scholar]
- Helenius A., Simons K. The binding of detergents to lipophilic and hydrophilic proteins. J Biol Chem. 1972 Jun 10;247(11):3656–3661. [PubMed] [Google Scholar]
- Ito A., Sato R. Purification by means of detergents and properties of cytochrome b5 from liver microsomes. J Biol Chem. 1968 Sep 25;243(18):4922–4923. [PubMed] [Google Scholar]
- Makino S., Reynolds J. A., Tanford C. The binding of deoxycholate and Triton X-100 to proteins. J Biol Chem. 1973 Jul 25;248(14):4926–4932. [PubMed] [Google Scholar]
- Maroux S., Louvard D., Baratti J. The aminopeptidase from hog intestinal brush border. Biochim Biophys Acta. 1973 Sep 15;321(1):282–295. doi: 10.1016/0005-2744(73)90083-1. [DOI] [PubMed] [Google Scholar]
- Maroux S., Louvard D. On the hydrophobic part of aminopeptidase and maltases which bind the enzyme to the intestinal brush border membrane. Biochim Biophys Acta. 1976 Jan 21;419(2):189–195. doi: 10.1016/0005-2736(76)90345-x. [DOI] [PubMed] [Google Scholar]
- POLLOCK M. R. PURIFICATION AND PROPERTIES OF PENICILLINASES FROM TWO STRAINS OF BACILLUS LICHENIFORMIS: A CHEMICAL, PHYSICOCHEMICAL AND PHYSIOLOGICAL COMPARISON. Biochem J. 1965 Mar;94:666–675. doi: 10.1042/bj0940666. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sawai T., Lampen J. O. Purification and characteristics of plasma membrane penicillinase from Bacillus licheniformis 749-C. J Biol Chem. 1974 Oct 10;249(19):6288–6294. [PubMed] [Google Scholar]
- Simons K., Helenius A., Garoff H. Solubilization of the membrane proteins from Semliki Forest virus with Triton X100. J Mol Biol. 1973 Oct 15;80(1):119–133. doi: 10.1016/0022-2836(73)90236-2. [DOI] [PubMed] [Google Scholar]
- Tanford C., Reynolds J. A. Characterization of membrane proteins in detergent solutions. Biochim Biophys Acta. 1976 Oct 26;457(2):133–170. doi: 10.1016/0304-4157(76)90009-5. [DOI] [PubMed] [Google Scholar]
- Visser L., Robinson N. C., Tanford C. The two-domain structure of cytochrome b5 in deoxycholate solution. Biochemistry. 1975 Mar 25;14(6):1194–1199. doi: 10.1021/bi00677a015. [DOI] [PubMed] [Google Scholar]