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. 1978 Jul;75(7):3273–3277. doi: 10.1073/pnas.75.7.3273

Identification, localization, and role of fibronectin in cultured bovine endothelial cells.

C R Birdwell, D Gospodarowicz, G L Nicolson
PMCID: PMC392757  PMID: 277924

Abstract

We have examined bovine aortic endothelial cell cultures for the presence of fibronectin, a high molecular weight cell-surface glycoprotein. Sparse cultures contain fibronectin only on dorsal cell surfaces at regions of cell-cell contact, as detected by immunofluorescence. In contrast, when the endothelial cells reached confluence as a highly contact-inhibited monolayer, fibronectin was detected in an extracellular matrix underneath the cell monolayer but not on top of the monolayer. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis of isolated extracellular matrix revealed that a predominant component of the matrix is a protein of approximately 2.3 X 10(5) molecular weight, which has been identified as fibronectin.

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