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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: Neuron. 2006 Oct 19;52(2):255–269. doi: 10.1016/j.neuron.2006.09.037

Figure 4. MeCP2 Is Phosphorylated at S421 Specifically in the Brain.

Figure 4

Western analysis of whole-cell lysates prepared from different mammalian tissues using antibodies specific to total MeCP2, MeCP2 pS421, and pCREB (S133). Fresh tissues were collected from a 4-week-old male mouse, directly homogenized into 1.5% SDS sample buffer at a ratio of 50 mg/ml w:v, and separated on a 12% SDS-PAGE gel. The same blot was reprobed with an anti-GAPDH antibody as an indicator of sample loading.