Figure 2.
MICU1 and MICU2 Dimerization
(A) HeLa cells were transfected with the indicated constructs or siRNAs. HeLa cells were harvested after 48 hr of transfection, and total protein was extracted and subjected to western blotting analysis with αMICU2 and αMICU1 antibodies. SDS-PAGE was performed in the presence or absence of DTT as indicated.
(B) Homo- or heterodimer formation of MICU1, MICU2, MICU1C465A, and MICU2C410A after overexpression. HeLa cells were harvested after 48 hr of transfection, and total protein was extracted and subjected to western blotting analysis with αFlag and αHA antibodies. SDS-PAGE was performed in the absence of DTT.
(C) HeLa cells were transfected with the indicated constructs. Flag-tagged MICU2 was immunoprecipitated from whole-cell lysate with a specific αFlag-agarose-conjugated antibody. The precipitated proteins were immunoblotted with αMCU and αFlag antibodies. SDS-PAGE was performed in the absence of DTT.
See also Figures S1 and S2.