Skip to main content
. 2014 Mar 6;53(5):726–737. doi: 10.1016/j.molcel.2014.01.013

Figure 5.

Figure 5

MICU2 Acts as an Inhibitor of MCU Activity

(A) [Ca2+]mt measurements in control and MICU2-silenced HeLa cells and challenged with maximal histamine stimulation.

(B) [Ca2+]mt measurements in intact HeLa control and MICU2-overexpressing cells.

(C and D) In vitro expressed MCU activity was recorded in sodium-gluconate containing 5 mM EDTA (10 pM free calculated [Ca2+]) at −40 mV Vcis. Upper panels show representative traces, whereas lower panels contain amplitude histograms that were obtained by analyses of 100 s long traces before (C) and after (D) addition of MICU2 to the cis side. A similar effect was observed in another four experiments with a decrease of the open probability by ≥70% upon addition of MICU2.

Data are presented as mean ± SD. Descriptive statistics can be found in Table S1. ∗p < 0.05 compared to control. See also Figures S4 and S5.