Figure 2. Candidate regulators for the specification of alternate epigenetic states in GBM.
(A) A set of 19 TPC-specific TFs is identified based on RNA-Seq expression and promoter H3K27ac signals in TPCs and DGCs. TF family is indicated at right. (B) Western blots confirm exclusive protein expression in TPCs for selected TFs. Lower panel indicates tubulin loading control. (C) ChIP-Seq tracks show H3K27ac signals for loci encoding TPC-specific TFs OLIG1, OLIG2 and SOX2, or (D) the differentiation factor BMP4 in the respective GBM models. TPC-specific TF loci are enriched for TPC-specific regulatory elements.