Table 2. Table Inbred C57BL/6J High and Low Dose STZ Models of DN.
| Model | Duration (weeks) |
STZ mg/kg |
Behavior | NCV | Anatomy | Biochemistry Gene Expression |
Glucose | Weight grams |
First Author Citation |
|---|---|---|---|---|---|---|---|---|---|
| High Dose | |||||||||
| C57BL/6J | 6 | 200 | NA | NA | ↓peptidergic input to spinal cord lamina IIi |
NA | ~25 mmol/L | ↓30% | Akkina Exp Neurol 167:173-182, 2001 |
| 7 | 180 | NA | NA | ↓IENF density | NA | 405 mg/dL | 21 | Christianson Exp Neurol 179:188-199, 2003 |
|
| 9 | 180 | no difference thermal or chemogenic stimuli; mechanical hypoalgesia |
NA | NA | NA | 292 mg/dL | 21 | Christianson J Pain 4:493-504, 2003 |
|
| 9 | 150 | NA | ↓MNCV ↓SNCV |
NA | ↓PCr/Cr ↑glucose ↑sorbitol ↑fructose |
23.4 mmol/L | 24.9 | Obrosova FASEB J 19:401-420, 2004 |
|
| 6 | 180 | no difference in mechanosensory function |
NA | ↓DRG neuronal size |
↑ATF3 expression |
26.6 mmol/L | 22.33 | Wright J Periph Nerv Syst 9:242-254, 2004 |
|
| 1 | 200 | ↓thermal sensitivity | NA | NA | NA | > 20 mmol/L | NA | Gabra Reg Pep 127:245-248, 2005 |
|
| 8 | 180 | ↓mechanical sensitivity | NA | ↓IENF | NA | 366 mg/dL | 21.3 | Christianson Neuroscience 145:303-313, 2007 |
|
| 7 | 180 | ↓formalin-induced pain behavior |
NA | ↓number of Fos positive neurons in dorsal horn |
NA | 20 mmol/L | 20 | Johnson J Pain 8:637-649, 2007 |
|
| 6 | 100 | ↓thermal sensitivity, ↓me- chanical sensitivity (pressure), ↑mechanical sensitivity (non Frey filament) |
NA | ↓IENF ↑NT IHC ↑PAR IHC |
NA | 33 mmol/L | 25 | Drel Europ J Pharm 569: 48-58, 2007 |
|
| Low Dose | |||||||||
| C57BL/6J | 12 | 40 /5 | ↑hind paw latency | NA | NA | ↓mitochondrial activity |
26.4 mmol/dL | 19.3 | Tam FASEB J 18:1767-1769, 2004 |
|
129/Sv X
C57Bl6 |
~7 | 40 / 7 | NA | ↓motor and sensory NCV |
↓nerve blood flow |
↓PCr, PCr/Cr | 22.7 mmol/L | 24.5 | Obrosova Diabetes 53:711-720, 2004 |
| B6/129S7 | 6 | 40 / 5 | NA | ↓motor and sensory NCV |
NA | ↓GSH, ↓SOD, ↑MDA, ↑catalase |
329 mg/dL | 19.7 | Kellogg Antiox and Redox Sig 7:1521-1529, 2005 |
| C57BL/6J | NA | 50 / 5 | NA | NA | NA | prolonged Ca++ elevation in DRG neurons |
21.15 mmol/L | NA | Kostyuk Neuroscience 90:535-541, 1999 |
| C57BL/6J | 24 | 50 / 5 | no change in thermal latency |
no change in NCV |
no change in IENF |
no change in GSH, SOD, MDA, Catalase |
366 mg/dL | Vincent Exper Neurol 208:216-227, 2007 |
|
| C57BL/6J | 24 | 50 / 5 | no change in thermal latency |
no change in NCV |
no change in TUNEL+ cells or IENF |
NA | 466 mg/dL | Sullivan Neurobiol Dis 28:276-285, 2007 |
|
ATF3 = activating transcription factor 3, IENF = intraepidermal nerve fiber, IHC = immunohistochemistry, GSH = glutathione S-transferase, MDA = malondialdehyde, NCV. = nerve conduction velocity, NT = nitrotyrosine, PAR = poly(ADP-ribose), PCr/Cr = phosphocreatinine/creatinine ratio, SOD = superoxide dismutase.