Table 3.
Overview of the hitstological and Immunohistochemical score
| Group | Inflammation (HE) | Goblet cells (PAS) | Connective tissue (Masson) | α-smooth muscle cells (anti-α-smooth muscle actin) | Proliferating cells (anti-Ki67) |
|---|---|---|---|---|---|
| OVA/Sal-PBS |
- |
- |
- |
+ |
- |
| OVA/OVA-PBS |
++ |
+ |
++ |
++ |
++ |
| OVA/Sal-B. breve |
- |
- |
+ |
+ |
+/- |
| OVA/OVA-B. breve |
+ |
+ |
+ |
++ |
+ |
| OVA/Sal-L. rhamnosus |
- |
- |
+ |
+ |
+/- |
| OVA/OVA-L. rhamnosus |
+ |
+ |
+ |
++ |
+/- |
| OVA/Sal-budesonide |
- |
- |
+ |
+ |
+/- |
| OVA/OVA-budesonide | + | - | + | ++ | + |
Slides were reviewed in blinded fashion by two observers independently and slides were scored on the basis of the percentage of positive stained cells in the following way: -,no positive staining; +/-, less than 25% of cells stained positive; +,25 to 50% cells stained positive; ++, 50 to 75% cells stained positive. Results are presented as average score of 5 animals/group.