Table 3.
Distribution of BES BLAST Hits in the Human Genome, Their Predicted Cattle Chromosome Locations, and Genome Coverage
| HSA | Chr. size (Mb) | No. hits | Predicted cattle chromosomes (no. hits) | No. in gaps (%) | % Coveragea |
|---|---|---|---|---|---|
| 1 | 246 | 1137 | BTA2 (95), BTA3 (581), BTA16 (230), BTA28 (19) | 212 (18.6) | 29.6 |
| 2 | 240 | 1279 | BTA2 (294), BTA11 (476) | 509 (39.8) | 35.5 |
| 3 | 194 | 999 | BTA1 (428), BTA22 (51) | 520 (52.0) | 34.2 |
| 4 | 192 | 827 | BTA6 (458), BTA17 (104) | 265 (32.0) | 31.3 |
| 5 | 181 | 936 | BTA7 (282), BTA20 (236) | 418 (44.6) | 34.6 |
| 6 | 170 | 795 | BTA9 (180), BTA23 (234) | 381 (47.9) | 34.9 |
| 7 | 157 | 716 | BTA4 (470), BTA25 (16) | 230 (32.1) | 33.8 |
| 8 | 143 | 635 | BTA8 (10), BTA14 (208), BTA27 (55) | 362 (57.0) | 32.8 |
| 9 | 132 | 552 | BTA8 (294), BTA11 (37) | 221 (40.0) | 32.1 |
| 10 | 134 | 652 | BTA13 (84), BTA26 (221), BTA28 (122) | 225 (34.5) | 34.9 |
| 11 | 137 | 673 | BTA15 (365), BTA29 (17) | 291 (43.2) | 34.5 |
| 12 | 131 | 611 | BTA5 (491), BTA17 (71) | 49 (8.0) | 34.3 |
| 13 | 113 | 397 | BTA12 (397) | 0 | 28.0 |
| 14 | 104 | 529 | BTA10 (229), BTA21 (51) | 249 (47) | 32.2 |
| 15 | 99 | 449 | BTA10 (227), BTA21 (108) | 114 (25.3) | 28.5 |
| 16 | 81 | 348 | BTA18 (27), BTA25 (110) | 166 (47.7) | 29.4 |
| 17 | 80 | 372 | BTA19 (372) | 0 | 36.6 |
| 18 | 77 | 342 | BTA24 (342) | 0 | 32.9 |
| 19 | 60 | 259 | BTA7 (91), BTA18 (64) | 104 (40.0) | 30.2 |
| 20 | 62 | 284 | BTA13 (284) | 0 | 31.8 |
| 21 | 44 | 153 | BTA1 (153) | 0 | 23.0 |
| 22 | 47 | 155 | BTA5 (43), BTA17 (53) | 59 (38.10 | 20.0 |
| X | 149 | 565 | BTAX (565) | 0 | 29.7 |
| Total | 2,973 | 13,665 | 4,375 (32.0) | 32.3 |
a
Coverage of human genome by cattle BACs was estimated by summing the lengths of human genome sequences between cattle BESs for which both ends matched the same human chromosome with a distance <300 Kbp and the number of BESs having only one high-confidence hit in the human genome at a distance ≥180 Kbp multiplied by 180 Kbp. The coverage within the cattle genome should be approximately the same, given that 91% of all paired-end cattle BESs with BLAST hits on the same chromosome were separated by <300 Kbp