Figure 4.
Quantitative measurement of organoid viability by MTT reduction. Intestinal crypts were isolated, counted and seeded in serial dilutions. Before measuring the relative MTT reduction at 562 nm, the number of organoids per visual field (using the × 10 objective) was counted by eye. MTT reduction was measured in SDS- and DMSO-solubilized organoids. (a) Correlation between theoretically seeded crypts and organoids counted per visual field. (b) Correlation between counted organoids per visual field and relative MTT reduction. (c) Correlation between theoretically seeded crypts and measured relative MTT reduction. Dotted lines indicate confidence intervals CI95% of best-fit line