Figure 5.
Quantitative measurement of organoid death in comparison to human and murine cancer cell lines. (a–c) MTT reduction assay was used for determining cell death in the organoid cultures. Crypt seeding density was normalized by resazurin reduction before treatment. Cell death was either induced by different doses of cisplatin or irinotecan (a), mTNFα (b) or X-ray irradiation (c). (d–f) Caco-2 cells were treated with different doses of either cisplatin or irinotecan (d), hTNFα±actinomycin D (60 ng/ml) (e), or X-ray irradiation (f) and cell death determined by MTT reduction. (g–i) MC38 cells were treated with different doses of either cisplatin or irinotecan (g), mTNFα±actinomycin D (60 ng/ml) (h) or X-ray irradiation (i) and cell death determined by MTT reduction. Each treatment was performed in triplicates. Mean values ±S.D. are shown. Data are representative for three independent experiments with comparable results