Table 1.
Primary antibodies
| Primary Antibody | Company | Species raised in | Catalogue Number | Section thickness (μm) | Heat-Induced Epitope Retrieval | Antibody dilution |
|---|---|---|---|---|---|---|
| Proliferating Cell Nuclear Antigen | Santa Cruz Biotechnology, California, USA | Mouse monoclonal | sc-56 | 48 | 10mM sodium citrate buffer pH 8.5 at 60°C overnight | 1:750 |
| 7 | 10mM Tris 1mM EDTA pH 9.0 at 95°C for 30 minutes | 1:500 | ||||
| Ionized calcium binding adaptor molecule 1 | Wako Pure Chemicals, Osaka, Japan | Rabbit polyclonal | 019-19741 | 48 | None required | 1:1000 |
| 7 | 10mM Tris 1mM EDTA pH 9.0 at 95°C for 30 minutes | 1:1000 | ||||
| Ki-67 (Clone MIB-1) | Dako, Denmark | Mouse monoclonal | M7240 | 7 | 10mM Tris 1mM EDTA pH 9.0 at 110°C for 30 minutes | 1:500 |
| Beta III Tubulin | Abcam, Cambridge, USA | Rabbit polyclonal | ab18207 | 7 | 10mM Tris 1mM EDTA pH 9.0 at 110°C for 30 minutes | 1:200 |
| GFAP | Dako, Denmark | Rabbit polyclonal | Z0334 | 7 | 10mM Tris 1mM EDTA pH 9.0 at 110°C for 30 minutes | 1:500 |
| NeuN | Millipore, Billerica, USA | Rabbit polyclonal | ABN78 | 7 | 10mM Tris 1mM EDTA pH 9.0 at 110°C for 30 minutes | 1:200 |