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. 2014 Jun 30;211(7):1289–1296. doi: 10.1084/jem.20132459

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© 2014 Min-Oo et al.

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Figure 4. — Bim deficiency alters the phenotype of memory NK cells. Ly49H⁺ memory NK cells generated by adoptive transfer into Ly49H-deficient mice were assessed at day 35 after MCMV. (A) Representative plots show the expression of CD11b and CD27 on WT and Bcl2l11^−/− Ly49H⁺ memory NK cells. (B) The proportions of CD11b⁻CD27⁺, CD11b⁺CD27⁻, and CD11b⁺CD27⁻Ly49H⁺ NK1.1⁺TCRβ⁻ NK cells are presented in a bar graph in uninfected mice and at days 8, 15, and 35 p.i. (C) The absolute number of Ly49H⁺ NK cells in each maturation state was enumerated in the spleen at day 35 after MCMV. (D) The percentage of DNAM-1⁺Ly49H⁺ NK cells was quantified in the Bcl2l11^−/− and WT compartment. (E) Representative plots showing the expression of KLRG1 and Ly6C on Bcl2l11^−/− (CD45.1) and WT (CD45.2) Ly49H⁺ memory NK cells are presented. Data in each panel are representative of two to three independent experiments. Error bars signify the standard error of the mean. ***, P < 0.001; *, P < 0.05.