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Mutational analysis of HO-1 gene. (a) Reverse transcriptase-PCR for HO-1 mRNA. M denotes molecular weight marker. Lane 1, control; lane 2, patient; lane 3, father; and lane 4, mother. (b) Sequence analysis of paternal allele and normal control allele. (c) Sequence analysis of maternal allele and normal control allele. (d) Mutation- specific PCR of genomic DNA. Data from control (lane 1), patient (lane 2), father (lane 3), and mother (lane 4) are shown. (e) Control PCR.
