Figure 1.
Strategy used to disrupt the mouse PLTP gene. (a) The top line represents the map of the endogenous murine PLTP gene and its flanking sequence. The middle line represents the vector used to target the PLTP locus. The bottom line shows the predicted organization of the locus after homologous recombination. A probe and a pair of PCR primers indicated in this line were used to confirm the integrity of site-specific integration. (b) Southern blot analysis of ES cells and (c) mouse tail-tip genomic DNA, respectively, digested with EcoRI and hybridized with the probe. Normal ES cell DNA and control mice DNA with 2.1-kb signal only (+/+); targeted ES cell DNA and heterozygous deficient mice with 2.1-kb and 1.8-kb signal (+/–); homozygous deficient mice with 1.8-kb signal only (–/–). ES, embryonic stem; neo, neomycin-resistant gene; PLTP, phospholipid transfer protein; tK, herpes simplex virus thymidine kinase gene.