FIG. 2.
Calibration curves from laboratories (labs 2–6) running either qRT-PCR (y-axis) or CLPA assay (lab 8, y-axis) are shown. Data points are fitted by a regression line of second order (labs 2–6) or connected with a spline curve (lab 8). Details are provided in parenthesis. Symbols represent mean values of triplicate measurements. Error bars represent the standard deviation and are visible when greater than the symbols. Calibration data for laboratories 1 and 7 were not reported. Either the equation or the calibration curves itself were used to calculate or extrapolate by hand corresponding dose estimates from the blinded test samples gene expression values. All laboratories ultimately only used one gene for their calibration curves as shown in Fig. 2, but one laboratory did use 4 genes (data not shown). “RD” = radiation dose (Gy).