Table 2.

Change in ratio of radioactive myosin heavy chain to light chain, during differentiation of primary muscle cultures.

Myosin extracted at various times following the change from FE medium to S medium was subjected after step A of purification (Table 1) to SDS-polyacrylamide gel electrophoresis and the radioactivity of the bands co-migrating with myosin heavy and light chains was measured as described in Methods